Positions

Overview

  • Current research and/or professional activities:
    Antifreeze proteins :
    Antifreeze proteins are found in a wide range of cold adapted organisms, and they contribute to their freeze resistance. Antifreeze proteins adsorb to the ice surface and inhibit the growth of ice crystals. The goal of this project is to investigate the mechanism by which antifreeze proteins protect against the damage typically inflicted by cold, including the underlying molecular mechanism of ice-binding. We also explore their applications in cryopreservation.

    Membrane proteins :
    Approximately one-third of all proteins are located in biological membranes. Membrane proteins have diverse biological functions, and it is estimated that they constitute at least half of all therapeutic drug targets. Structural elucidation of membrane proteins remains challenging, and less than 2% are structurally characterized. We utilize both solution and solid state NMR techniques to investigate membrane proteins in solution and in their native environment, in membranes.

    Chiral nanoparticles:
    Quantum dots are nanometer size semiconductor crystals with excellent and tunable electronic and optical properties. Colloidal quantum dots consist of an inorganic semiconductor core (e.g. CdSe) and an organic capping ligand shell (e.g. cysteine). The objective of this study is to determine how chiral organic ligands induce chiroptical activity in achiral semiconductor QDs and how QDs can be used to enhance the chiroptical signal of biomolecules. Chiral QDs are promising candidates for bioimaging, biosensing, environmental nanoassays, catalysis, and chiral memory.
  • Publications

    Academic Article

    Year Title
    2018 Changes in Cryopreserved Cell Nuclei Serve as Indicators of Processes during Freezing and Thawing.Langmuir2018
    2018 Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are differentially controlled by cryoprotectants.Scientific Reports.  8:14694. 2018
    2017 CdSe Quantum Dots Functionalized with Chiral, Thiol-Free Carboxylic Acids: Unraveling Structural Requirements for Ligand-Induced Chirality.ACS Nano.  11:9846-9853. 2017
    2017 Organic coating on biochar explains its nutrient retention and stimulation of soil fertility.Nature Communications.  8:1089. 2017
    2017 Chiral multichromophoric supramolecular nanostructures assembled by single stranded DNA and RNA templatesCoordination Chemistry Reviews.  349:66-83. 2017
    2017 Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethyl sulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability.RSC Advances.  7:352-360. 2017
    2016 The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy.Biochemistry and Biophysics Reports.  8:75-80. 2016
    2016 Caulobacter PopZ forms an intrinsically disordered hub in organizing bacterial cell poles.Proceedings of the National Academy of Sciences of the United States of America.  113:12490-12495. 2016
    2016 Chirality Inversion of CdSe and CdS Quantum Dots without Changing the Stereochemistry of the Capping Ligand.ACS Nano.  10:3809-3815. 2016
    2015 ¹³C- and ¹H-detection under fast MAS for the study of poorly available proteins: application to sub-milligram quantities of a 7 trans-membrane protein.Journal of Biomolecular NMR.  62:17-23. 2015
    2014 Characterization of the mechanism of gasification of a powder river basin coal with a composite catalyst for producing desired syngases and liquidsApplied Catalysis A: General.  475:116-126. 2014
    2013 Ligand induced circular dichroism and circularly polarized luminescence in CdSe quantum dots.ACS Nano.  7:11094-11102. 2013
    2013 Achiral CdSe quantum dots exhibit optical activity in the visible region upon post-synthetic ligand exchange with D- or L-cysteine.Chemical Communications.  49:1844-1846. 2013
    2012 Targeting the lateral interactions of transmembrane domain 5 of Epstein-Barr virus latent membrane protein 1.Biochimica et Biophysica Acta - Biomembranes Biochimica et Biophysica Acta - Reviews on Biomembranes.  1818:2282-2289. 2012
    2011 The conformation of bacteriorhodopsin loops in purple membranes resolved by solid-state MAS NMR spectroscopy.Angewandte Chemie Angewandte Chemie - International Edition.  50:8432-8435. 2011
    2011 Structure of the BamC two-domain protein obtained by Rosetta with a limited NMR data set.Journal of Molecular Biology.  411:83-95. 2011
    2008 Advances towards resonance assignments for uniformly--13C, 15N enriched bacteriorhodopsin at 18.8 T in purple membranes.Journal of Biomolecular NMR.  41:1-4. 2008
    2007 Solid-state NMR study and assignments of the KcsA potassium ion channel of S. lividans.Biochimica et Biophysica Acta - Proteins and Proteomics Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology.  1774:1604-1613. 2007

    Conference Proceeding

    Year Title
    2015 Origin and tuning of chirality in quantum dotsACS National Meeting Book of Abstracts. 2015
    2015 Structural and functional characterization of the antifreeze protein ApAFP752ACS National Meeting Book of Abstracts. 2015
    2015 Structural and functional studies of TSPOACS National Meeting Book of Abstracts. 2015

    Teaching Activities

  • Doctoral Thesis Taught course 2019
  • General Biochemistry Taught course 2019
  • Physical Biochemistry Taught course 2019
  • Doctoral Thesis Taught course 2018
  • Intro to Research in Life Sci Taught course 2018
  • Principles of Biochemistry I Taught course 2018
  • Doctoral Research Taught course 2018
  • Doctoral Research Taught course 2018
  • Physical Biochemistry Taught course 2018
  • Doctoral Research Taught course 2017
  • General Biochemistry Taught course 2017
  • General Biochemistry Taught course 2017
  • Intro to Research in Life Sci Taught course 2017
  • Doctoral Research Taught course 2017
  • Physical Biochemistry Taught course 2017
  • Doctoral Research Taught course 2016
  • Intro to Research in Life Sci Taught course 2016
  • Education And Training

    Full Name

  • Krisztina Varga