Positions

Overview

  • The Varga lab focuses on the biophysical characterization of protein structure and function. We use NMR and other spectroscopic techniques to investigate protein structure, protein-protein, and protein-ligand interactions. Another major research interest is the design and characterization of chiral nanoparticles. Ongoing projects include:

    ANTIFREEZE PROTEINS
    Antifreeze proteins are found in a wide range of cold adapted organisms, and they contribute to their freeze resistance. Antifreeze proteins adsorb to the ice surface and inhibit the growth of ice crystals. The goal of this project is to investigate the mechanism by which antifreeze proteins protect against the damage typically inflicted by cold, including the underlying molecular mechanism of ice-binding. This project is supported by NASA-EPSCoR and NIH.

    CHIRAL NANOPARTICLES
    Quantum dots (QDs) are nanometer size semiconductor crystals with excellent and tunable electronic and optical properties. Colloidal quantum dots consist of an inorganic semiconductor core (e.g. CdSe) and an organic capping ligand shell (e.g. cysteine). We aim to determine how chiral organic ligands induce chiroptical activity in achiral semiconductor QDs and how QDs can be used to enhance the chiroptical signal of biomolecules. Chiral QDs are promising candidates for bioimaging, biosensing, environmental nanoassays, catalysis, and chiral memory. his project was supported by NSF.

    REGULATORS OF G-PROTEIN SIGNALING PROTEINS
    The discoveries of a class of intracellular regulatory proteins known as regulators of G-protein signaling (RGS) proteins that mediate GPCR signaling via protein-protein interactions between the RGS domains and the gamma-subunit of G-proteins and their covalent inhibitors have opened a new venue for allosteric targeting in GPCRs. We are studying inhibitor-induced structural perturbations using NMR and MD analyses of the RGS8 protein and its mutant forms to understand the role of cysteine residues in affecting potency and specificity of inhibitors. This project is supported by NIH.

    RATES OF PROTEIN EVOLUTION
    We aim to identify the factors that have an important impact on the rates of protein evolution and elucidate the reasons why these factors affect rates of evolution. During evolution, different proteins accumulate amino acid changes at enormously different rates as a result of the different selective pressures to which they are subjected. This project is supported by NSF.

    BACTERIAL MECHANISMS IN ESTABLISHING AND MAINTAINING CELL POLARITY
    The polar organizing protein Z (PopZ) is necessary for the formation of three-dimensional microdomains at the cell poles in Caulobacter crescentus, where it functions as a hub protein that recruits multiple regulatory proteins from the cytoplasm. Although a large portion of the protein is predicted to be natively unstructured, in reconstituted systems PopZ can self-assemble into a macromolecular scaffold that directly binds to at least ten different proteins. We have utilized NMR spectroscopy to study its interaction with its binding partners. This project was supported by NIH.
  • Co-principal Investigator On

    Teaching Activities

  • Doctoral Research Taught course
  • Doctoral Research Taught course
  • Honors Senior Thesis Taught course
  • Principles of Biochemistry Taught course
  • Principles of Biochemistry I Taught course
  • Principles of Biochemistry II Taught course
  • Principles of Biochemistry II Taught course
  • Doctoral Thesis Taught course 2023
  • Doctoral Thesis Taught course 2022
  • Principles of Biochemistry Taught course 2022
  • Principles of Biochemistry I Taught course 2022
  • Doctoral Thesis Taught course 2022
  • General Biochemistry Taught course 2022
  • General Biochemistry Taught course 2022
  • Honors Senior Thesis Taught course 2022
  • Student Research Experience Taught course 2022
  • Doctoral Thesis Taught course 2021
  • Honors Senior Thesis Taught course 2021
  • Principles of Biochemistry I Taught course 2021
  • Doctoral Thesis Taught course 2021
  • Honors Senior Thesis Taught course 2021
  • Physical Biochemistry Taught course 2021
  • Doctoral Thesis Taught course 2020
  • Honors Senior Thesis Taught course 2020
  • Principles of Biochemistry I Taught course 2020
  • Doctoral Thesis Taught course 2020
  • Physical Biochemistry Taught course 2020
  • Doctoral Thesis Taught course 2019
  • Principles of Biochemistry I Taught course 2019
  • Doctoral Thesis Taught course 2019
  • General Biochemistry Taught course 2019
  • Physical Biochemistry Taught course 2019
  • Doctoral Thesis Taught course 2018
  • Intro to Research in Life Sci Taught course 2018
  • Principles of Biochemistry I Taught course 2018
  • Doctoral Research Taught course 2018
  • Doctoral Research Taught course 2018
  • Physical Biochemistry Taught course 2018
  • Doctoral Research Taught course 2017
  • General Biochemistry Taught course 2017
  • General Biochemistry Taught course 2017
  • Intro to Research in Life Sci Taught course 2017
  • Doctoral Research Taught course 2017
  • Physical Biochemistry Taught course 2017
  • Doctoral Research Taught course 2016
  • Intro to Research in Life Sci Taught course 2016
  • Education And Training

  • B.S. Chemistry, St. John’s University
  • M.S. Biology, St. John’s University
  • Ph.D. Chemistry, Columbia University
  • Full Name

  • Krisztina Varga