Positions

Research Areas research areas

Publications

Academic Article

Year Title
2019 The N termini of the inhibitory γ-subunits of phosphodiesterase-6 (PDE6) from rod and cone photoreceptors differentially regulate transducin-mediated PDE6 activation.Journal of Biological Chemistry.  jbc.RA119.007520-jbc.RA119.007520. 2019
2019 Pharmacological and molecular dynamics analyses of differences in inhibitor binding to human and nematode PDE4: Implications for management of parasitic nematodes.PLoS ONE.  14:e0214554. 2019
2014 Molecular architecture of photoreceptor phosphodiesterase elucidated by chemical cross-linking and integrative modeling.Journal of Molecular Biology.  426:3713-3728. 2014
2013 PDE inhibitors as potential pesticides targeting parasitic nematodesFASEB Journal.  27. 2013
2013 Probing the Structure of Photoreceptor Phosphodiesterase (PDE6) and Its Interactions with Transducin by Cross-linking and Molecular ModelingFASEB Journal.  27. 2013
2013 Proteomic Identification of Preferential Interactions between PDE6 and its Inhibitory SubunitFASEB Journal.  27. 2013
2013 Regulation of photoreceptor phosphodiesterase (PDE6) by the rod photoreceptor-specific glutamic acid-rich protein 2 (GARP2)FASEB Journal.  27. 2013
2012 Identification of amino acid residues responsible for the selectivity of tadalafil binding to two closely related phosphodiesterases, PDE5 and PDE6.Journal of Biological Chemistry.  287:41406-41416. 2012
2012 Functional mapping of interacting regions of the photoreceptor phosphodiesterase (PDE6) γ-subunit with PDE6 catalytic dimer, transducin, and regulator of G-protein signaling9-1 (RGS9-1).Journal of Biological Chemistry.  287:26312-26320. 2012
2012 Characterization of conformational changes and protein-protein interactions of rod photoreceptor phosphodiesterase (PDE6).Journal of Biological Chemistry.  287:20111-20121. 2012
2011 Phosphodiesterase 6C, cGMP-specific cone alpha'.AFCS Nat Mol Pages.  2011. 2011
2011 Phosphodiesterase 6D, cGMP-specific rod delta.AFCS Nat Mol Pages.  2011. 2011
2010 Structural requirements of the photoreceptor phosphodiesterase gamma-subunit for inhibition of rod PDE6 holoenzyme and for its activation by transducin.Journal of Biological Chemistry.  285:4455-4463. 2010
2009 Probing the catalytic sites and activation mechanism of photoreceptor phosphodiesterase using radiolabeled phosphodiesterase inhibitors.Journal of Biological Chemistry.  284:31541-31547. 2009
2008 Direct allosteric regulation between the GAF domain and catalytic domain of photoreceptor phosphodiesterase PDE6.Journal of Biological Chemistry.  283:29699-29705. 2008
2006 The glutamic acid-rich protein-2 (GARP2) is a high affinity rod photoreceptor phosphodiesterase (PDE6)-binding protein that modulates its catalytic properties.Journal of Biological Chemistry.  281:5500-5505. 2006
2005 Efficacy and selectivity of phosphodiesterase-targeted drugs in inhibiting photoreceptor phosphodiesterase (PDE6) in retinal photoreceptors.Investigative Ophthalmology and Visual Science.  46:3060-3066. 2005
2005 Rod and cone opsin families differ in spectral tuning domains but not signal transducing domains as judged by saturated evolutionary trace analysis.Journal of Molecular Evolution.  61:75-89. 2005
2005 cGMP signaling in vertebrate retinal photoreceptor cells.Frontiers in Bioscience - Landmark.  10:1191-1204. 2005
2005 Evaluation of the 17-kDa prenyl-binding protein as a regulatory protein for phototransduction in retinal photoreceptors.Journal of Biological Chemistry.  280:1248-1256. 2005
2005 Assay and functional properties of PrBP(PDEdelta), a prenyl-binding protein interacting with multiple partners.Methods in Enzymology.  403:42-56. 2005
2005 Cyclic guanosine 5'-monophosphate binding to regulatory GAF domains of photoreceptor phosphodiesterase.Methods in Molecular Biology.  307:141-154. 2005
2005 Purification of PDE6 isozymes from mammalian retina.Methods in Molecular Biology.  307:125-140. 2005
2004 Characteristics of photoreceptor PDE (PDE6): similarities and differences to PDE5.International Journal of Impotence Research.  16 Suppl 1:S28-S33. 2004
2003 Characterization of rod photoreceptor Glutamic Acid Rich Protein interaction with rod photoreceptor PDE6Investigative Ophthalmology and Visual Science.  44:U135-U135. 2003
2003 Relative potency of various classes of phosphodiesterase (PDE) inhibitors for rod and cone photoreceptor PDEInvestigative Ophthalmology and Visual Science.  44:U357-U357. 2003
2002 Regulation of cone photoreceptor phosphodiesterase (PDE6C) by its inhibitory gamma ' subunit and by cGMP bindingInvestigative Ophthalmology and Visual Science.  43:U451-U451. 2002
2002 Regulation of rod photoreceptor phosphodiesterase (PDE6) by phosphorylation of its catalytic subunitsInvestigative Ophthalmology and Visual Science.  43:U318-U318. 2002
2002 Regulation of photoreceptor phosphodiesterase (PDE6) by phosphorylation of its inhibitory gamma subunit re-evaluated.Journal of Biological Chemistry.  277:5017-5023. 2002
2001 Molecular organization of bovine rod cGMP-phosphodiesterase 6.Journal of Molecular Biology.  310:781-791. 2001
2001 The catalytic and GAF domains of the rod cGMP phosphodiesterase (PDE6) heterodimer are regulated by distinct regions of its inhibitory gamma subunit.Journal of Biological Chemistry.  276:27527-27534. 2001
2001 Characterization of the membrane-associated photoreceptor phosphodiesterase (PDE) following detergent solubilization from rod outer segment membranes.Investigative Ophthalmology and Visual Science.  42:S186-S186. 2001
2001 Effect of phosphorylation of the inhibitory gamma subunit (P gamma) on cGMP binding and catalytic activity of bovine rod photoreceptor phosphodiesterase (PDE)Investigative Ophthalmology and Visual Science.  42:S186-S186. 2001
2001 Multiple sites of high affinity interaction of the inhibitory gamma subunit (P gamma) with rod photoreceptor phosphodiesterase (PDE6).Investigative Ophthalmology and Visual Science.  42:S186-S186. 2001
2001 Solubilization of membrane-associated frog phosphodiesterase (PDE) in vitro by the frog homologue of the bovine 17 kDa delta protein.Investigative Ophthalmology and Visual Science.  42:S187-S187. 2001
2000 Mechanism of transducin activation of frog rod photoreceptor phosphodiesterase. Allosteric interactiona between the inhibitory gamma subunit and the noncatalytic cGMP-binding sites.Journal of Biological Chemistry.  275:38611-38619. 2000
2000 Identifying domains on the inhibitory gamma subunit (P gamma) that regulate bovine rod photoreceptor phosphodiesterase (PDE) function.Investigative Ophthalmology and Visual Science.  41:S606-S606. 2000
2000 Subcellular localization of frog rod photoreceptor phosphodiesterase (PDE) subunits.Investigative Ophthalmology and Visual Science.  41:S606-S606. 2000
2000 Transducin activation of photoreceptor phosphodiesterase: effects on catalytic activity, cGMP binding affinity to the noncatalytic sites, and PDE gamma subunit dissociation from the catalytic alpha beta dimer.Investigative Ophthalmology and Visual Science.  41:S606-S606. 2000
2000 Kinetics and regulation of cGMP binding to noncatalytic binding sites on photoreceptor phosphodiesterase.Methods in Enzymology.  315:646-672. 2000
1999 cGMP binding to noncatalytic sites on mammalian rod photoreceptor phosphodiesterase is regulated by binding of its gamma and delta subunits.Journal of Biological Chemistry.  274:18813-18820. 1999
1999 Regulation of photoreceptor phosphodiesterase catalysis by its non-catalytic cGMP-binding sites.Biochemical Journal.  340 ( Pt 3):863-869. 1999
1999 Potency and mechanism of action of E4021, a type 5 phosphodiesterase isozyme-selective inhibitor, on the photoreceptor phosphodiesterase depend on the state of activation of the enzyme.Molecular Pharmacology.  55:508-514. 1999
1998 Structural features of the noncatalytic cGMP binding sites of frog photoreceptor phosphodiesterase using cGMP analogs.Journal of Biological Chemistry.  273:5557-5565. 1998
1998 Photoreceptor phosphodiesterase: interaction of inhibitory gamma subunit and cyclic GMP with specific binding sites on catalytic subunits.Methods Neuroprotocols.  14:93-104. 1998
1997 Regulation of the binding affinity and rate of dissociation of cGMP at noncatalytic binding sites on photoreceptor phosphodiesteraseInvestigative Ophthalmology and Visual Science.  38:103-103. 1997
1997 The PDE inhibitor E4021 has complex effects on the frog rod photoreceptor phosphodiesteraseInvestigative Ophthalmology and Visual Science.  38:98-98. 1997
1995 KINETICS OF CGMP BINDING TO NONCATALYTIC BINDING-SITES ON FROG ROD PHOTORECEPTOR PHOSPHODIESTERASEInvestigative Ophthalmology and Visual Science.  36:S642-S642. 1995
1995 STRUCTURAL FEATURES OF THE NONCATALYTIC CGMP BINDING-SITES IN FROG ROD PHOSPHODIESTERASE DETERMINED WITH CGMP ANALOGSInvestigative Ophthalmology and Visual Science.  36:S269-S269. 1995
1994 cGMP binding sites on photoreceptor phosphodiesterase: role in feedback regulation of visual transduction.Proceedings of the National Academy of Sciences of the United States of America.  91:4845-4849. 1994
1994 Regulation of intracellular cyclic GMP concentration by light and calcium in electropermeabilized rod photoreceptors.Journal of General Physiology.  103:67-86. 1994
1993 A centrifugal separation procedure detects moderate affinity cGMP binding sites in membrane-associated proteins and permeabilized cells.Analytical Biochemistry.  215:159-161. 1993
1993 Intracellular cGMP concentration in rod photoreceptors is regulated by binding to high and moderate affinity cGMP binding sites.Journal of Biological Chemistry.  268:17190-17198. 1993
1992 NUCLEOTIDE ENERGY-METABOLISM IN ELECTROPERMEABILIZED ROD PHOTORECEPTORSInvestigative Ophthalmology and Visual Science.  33:1008-1008. 1992
1992 LIGHT-INDUCED-CHANGES IN CGMP METABOLISM IN ELECTROPORATED RETINAL RODSFASEB Journal.  6:A519-A519. 1992
1992 Mechanisms of amplification, deactivation, and noise reduction in invertebrate photoreceptors.Society of General Physiologists Series.  47:175-199. 1992
1989 Cyclic GMP levels and membrane current during onset, recovery, and light adaptation of the photoresponse of detached frog photoreceptors.Journal of Biological Chemistry.  264:15384-15391. 1989
1989 Characterization of guanylate cyclase in squid photoreceptors.Visual Neuroscience.  3:1-7. 1989
1986 CHANGES IN CGMP CONCENTRATION CORRELATE WITH SOME, BUT NOT ALL, ASPECTS OF THE LIGHT-REGULATED CONDUCTANCE OF FROG ROD PHOTORECEPTORSJournal of Biological Chemistry.  261:2965-2975. 1986
1986 Changes in cGMP concentration correlate with some, but not all, aspects of the light-regulated conductance of frog rod photoreceptors.Journal of Biological Chemistry.  261:12965-12975. 1986
1986 cGMP influences guanine nucleotide binding to frog photoreceptor G-protein.Journal of Biological Chemistry.  261:313-318. 1986
1984 Light-induced decreases in cGMP concentration precede changes in membrane permeability in frog rod photoreceptors.Journal of Biological Chemistry.  259:9635-9641. 1984
1981 Formulation of the general rate equation for subunit flux at steady-state.Journal of Molecular Biology.  150:599-602. 1981
1981 Head-to-tail polymerization of microtubules in vitro.Journal of Molecular Biology.  150:577-599. 1981
1977 Release of Escherichia coli DNA from membrane complexes by single-strand endonucleases.Proceedings of the National Academy of Sciences of the United States of America.  74:2756-2760. 1977
1974 Isolation and characterization of rifampin-resistant and streptolydigin-resistant mutants of Bacillus subtilis with altered sporulation properties.Journal of Bacteriology.  120:253-265. 1974

Chapter

Year Title
2005 Purification of PDE6 Isozymes From Mammalian Retina.  125-140. 2005

Conference Proceeding

Year Title
2016 NEMATODE PHOSPHODIESTERASES ARE PROMISING TARGETS FOR NOVEL NEMATICIDESJournal of Nematology. 368-369. 2016
2014 Characterization of retinal phosphodiesterase: Dependence of activity on zinc and cadmium concentrationACS National Meeting Book of Abstracts. 2014
2005 Drugs targeting specific phosphodiesterase families: Selectivity toward photoreceptor PDE6 and effects on intact photoreceptorsInvestigative Ophthalmology and Visual Science. 2005
1987 GUANYLATE-CYCLASE ACTIVITY IN SQUID PHOTORECEPTOR-MEMBRANESBiophysical Journal. A269-A269. 1987
1985 INVOLVEMENT OF CGMP IN PHOTOTRANSDUCTION BY ISOLATED FROG RODS IN THE ABSENCE OF TRANSMEMBRANE CALCIUM GRADIENTSBiophysical Journal. A100-A100. 1985
1984 KINETIC MEASUREMENT OF CGMP CHANGES IN INTACT PHOTORECEPTORSBiophysical Journal. A1-A1. 1984
1980 HEAD-TO-TAIL POLYMERIZATION OF MICROTUBULES INVITROFASEB Journal. 1818-1818. 1980

Patents And Disclosures

Year Title
Methods of Identification and Use of Nematicide Compounds

Teaching Activities

  • Doctoral Thesis Taught course 2019
  • Doctoral Thesis Taught course 2018
  • Doctoral Research Taught course 2018
  • Doctoral Research Taught course 2018
  • Doctoral Research Taught course 2017
  • Rsrch Exp/MCBS Taught course 2017
  • Doctoral Research Taught course 2017
  • Doctoral Research Taught course 2017
  • Doctoral Research Taught course 2016
  • Tissue Engineering Taught course 2016
  • Tissue Engineering Taught course 2016
  • Doctoral Research Taught course 2016
  • Doctoral Research Taught course 2015
  • Doctoral Research Taught course 2015
  • Doctoral Research Taught course 2014
  • Doctoral Research Taught course 2014
  • Seminar Taught course 2014
  • Seminar Taught course 2014
  • Seminar/Integrated Physiology Taught course 2014
  • Education And Training

  • B.S. Biology-Chemistry, Tufts University
  • Ph.D. Molecular Biology, University of Wisconsin
  • Full Name

  • Rick Cote
  • Mailing Address

  • University of New Hampshire

    Molecular, Cellular, and Biomedical Sciences

    46 College Rd

    Durham, NH  03824

    United States