Prenatal protein malnutrition decreases KCNJ3 and 2DG activity in rat prefrontal cortex.

Academic Article


  • Prenatal protein malnutrition (PPM) in rats causes enduring changes in brain and behavior including increased cognitive rigidity and decreased inhibitory control. A preliminary gene microarray screen of PPM rat prefrontal cortex (PFC) identified alterations in KCNJ3 (GIRK1/Kir3.1), a gene important for regulating neuronal excitability. Follow-up with polymerase chain reaction and Western blot showed decreased KCNJ3 expression in the PFC, but not hippocampus or brainstem. To verify localization of the effect to the PFC, baseline regional brain activity was assessed with (14)C-2-deoxyglucose. Results showed decreased activation in the PFC but not hippocampus. Together these findings point to the unique vulnerability of the PFC to the nutritional insult during early brain development, with enduring effects in adulthood on KCNJ3 expression and baseline metabolic activity.
  • Authors

  • Amaral, AC
  • Jakovcevski, M
  • McGaughy, Jill
  • Calderwood, SK
  • Mokler, DJ
  • Rushmore, RJ
  • Galler, JR
  • Akbarian, SA
  • Rosene, DL
  • Status

    Publication Date

  • February 12, 2015
  • Published In

  • Neuroscience  Journal
  • Keywords

  • 2-deoxyglucose
  • Animals
  • Deoxyglucose
  • Down-Regulation
  • Female
  • G Protein-Coupled Inwardly-Rectifying Potassium Channels
  • GIRK1
  • Gene Expression
  • KCNJ3
  • Male
  • Malnutrition
  • Prefrontal Cortex
  • Pregnancy
  • Prenatal Nutritional Physiological Phenomena
  • Rats
  • Rats, Long-Evans
  • brain activity
  • prefrontal cortex
  • prenatal protein malnutrition
  • Digital Object Identifier (doi)

    Start Page

  • 79
  • End Page

  • 86
  • Volume

  • 286