Satellite-derived vegetation indices (VIs) have been widely used to approximate or estimate gross primary productivity (GPP). However, it remains unclear how the VI-GPP relationship varies with indices, biomes, timescales, and the bidirectional reflectance distribution function (BRDF) effect. We examined the relationship between VIs and GPP for 121 FLUXNET sites across the globe and assessed how the VI-GPP relationship varied among a variety of biomes at both monthly and annual timescales. We used three widely-used VIs: normalized difference vegetation index (NDVI), enhanced vegetation index (EVI), and 2-band EVI (EVI2) as well as a new VI - NIRV and used surface reflectance both with and without BRDF correction from the moderate resolution imaging spectroradiometer (MODIS) to calculate these indices. The resulting traditional (NDVI, EVI, EVI2, and NIRV) and BRDF-corrected (NDVIBRDF, EVIBRDF, EVI2BRDF, and NIRV, BRDF) VIs were used to examine the VI-GPP relationship. At the monthly scale, all VIs were moderate or strong predictors of GPP, and the BRDF correction improved their performance. EVI2BRDF and NIRV, BRDF had similar performance in capturing the variations in tower GPP as did the MODIS GPP product. The VIs explained lower variance in tower GPP at the annual scale than at the monthly scale. The BRDF-correction of surface reflectance did not improve the VI-GPP relationship at the annual scale. The VIs had similar capability in capturing the interannual variability in tower GPP as MODIS GPP. VIs were influenced by temperature and water stresses and were more sensitive to temperature stress than to water stress. VIs in combination with environmental factors could improve the prediction of GPP than VIs alone. Our findings can help us better understand how the VI-GPP relationship varies among indices, biomes, and timescales and how the BRDF effect influences the VI-GPP relationship.