Proliferative action of erythropoietin is associated with rapid protein tyrosine phosphorylation in responsive B6SUt.EP cells.

Academic Article


  • Erythropoietin is a prime regulator of the growth and terminal differentiation of erythroid blood cells. However, little is understood concerning its molecular mechanism of action. Presently it is shown in the responsive, factor-dependent murine cell line B6SUt.EP that erythropoietin induces the tyrosine phosphorylation of six plasma membrane-associated proteins in a time- and concentration-dependent fashion (i.e. phosphoproteins PY153, PY140, PY100, PY93, PY74, and PY54). Among these, PY153 was prominent. For all proteins, maximal levels of phosphorylation were induced within 3-7 min at low factor concentrations (100-500 pM). These findings establish tyrosine kinase activation as a novel candidate pathway of erythropoietin-induced proliferation. In addition, the tyrosine phosphorylation of six proteins with identical Mr, as well as a Mr 104,000 protein, was induced in B6SUt.EP cells by interleukin 3. In contrast, no induced tyrosine phosphorylation was detectable in the erythropoietin-responsive, leukemic erythroid cell line. Rauscher Red 1, yet proteins of Mr 153,000 and 54,000 were shown to be phosphorylated constitutively at relative levels greater than those observed in B6SUt.EP cells. A possible role for these phosphoproteins in hematopoietic cell transformation is considered.
  • Authors

  • Quelle, FW
  • Wojchowski, Don
  • Status

    Publication Date

  • January 5, 1991
  • Published In


  • Animals
  • Cell Division
  • Cell Line
  • Cell Membrane
  • Clone Cells
  • Erythropoietin
  • Interleukin-3
  • Kinetics
  • Membrane Proteins
  • Mice
  • Phosphoproteins
  • Tyrosine
  • Pubmed Id

  • 1985918
  • Start Page

  • 609
  • End Page

  • 614
  • Volume

  • 266
  • Issue

  • 1