Transcription factor binding to a DNA zip code controls interchromosomal clustering at the nuclear periphery.

Academic Article


  • Active genes in yeast can be targeted to the nuclear periphery through interaction of cis-acting "DNA zip codes" with the nuclear pore complex. We find that genes with identical zip codes cluster together. This clustering was specific; pairs of genes that were targeted to the nuclear periphery by different zip codes did not cluster together. Insertion of two different zip codes (GRS I or GRS III) at an ectopic site induced clustering with endogenous genes that have that zip code. Targeting to the nuclear periphery and interaction with the nuclear pore is a prerequisite for gene clustering, but clustering can be maintained in the nucleoplasm. Finally, we find that the Put3 transcription factor recognizes the GRS I zip code to mediate both targeting to the NPC and interchromosomal clustering. These results suggest that zip-code-mediated clustering of genes at the nuclear periphery influences the three-dimensional arrangement of the yeast genome.
  • Authors

  • Brickner, Donna Garvey
  • Ahmed, Sara
  • Meldi, Lauren
  • Thompson, Abbey
  • Light, Will
  • Young, Matthew
  • Hickman, Taylor L
  • Chu, Feixia
  • Fabre, Emmanuelle
  • Brickner, Jason H
  • Status

    Publication Date

  • June 12, 2012
  • Published In

  • Developmental Cell  Journal
  • Keywords

  • Chromosomes, Fungal
  • DNA
  • Gene Expression Regulation, Fungal
  • Glycine-tRNA Ligase
  • Multigene Family
  • Nuclear Pore
  • Promoter Regions, Genetic
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Digital Object Identifier (doi)

    Start Page

  • 1234
  • End Page

  • 1246
  • Volume

  • 22
  • Issue

  • 6