Positions

Overview

  • Glycosylation is one of the most frequent post-translational modifications. Interest in the biological roles of lipid and protein glycosylation has intensified because of increasing examples of their importance as recognition determinants. It has been estimated that about 0.5-1.0% of the translated mammalian genome participates in oligosaccharide production and function and many specific roles have now been documented to involve glycan structures. Four areas of research that have received considerable attention are (i) development and differentiation, (ii) cell adhesion and inflammation, (iii) cancer and metastasis, and (iv) host-pathogen interactions in infection diseases.

    The biosynthesis of glycans is catalyzed by glycosyltransferases, a family of 100 or more enzymes which transfer a sugar residue from a nucleotide-sugar donor to an acceptor which can be a sugar, an amino acid, or a lipid. These glycosyltransferases are classified on the basis of the carbohydrate residue they transfer, (e.g., galactosyltransferases, sialyltransferases, fucosyltransferases), and are distinguished by the sugar they recognize as an acceptor. Two fundamental features make structural characterization of these materials extremely difficult: product structures are frequently branched with considerable isomerism, and individual moieties are controlled at the level of transferase expression (not a direct product to be identified from the genome).

    Work in our laboratory has focused strongly on methods development in past years and more recently on application of these strategies and techniques to relate carbohydrate detailed structure to function. For reasons of sensitivity and specificity these methods depend heavily on mass spectrometry and their adjunct techniques. Latest studies have involved the use of ion trapping to determine carbohydrate structures where specific ions are selected for activation, collision, and measurement multiple times, MSn. This ability to isolate substructures for further characterization has become of vital importance to understand the detailed linkage and branching patterns inherent in lipid and protein glycosylation.
  • Publications

    Academic Article

    Year Title
    2018 Structures of N-Glycans of Bothrops Venoms Revealed as Molecular Signatures that Contribute to Venom Phenotype in Viperid Snakes.Molecular and Cellular Proteomics.  17:1261-1284. 2018
    2017 Isomeric complexity of glycosylation documented by MSn.Analytical and Bioanalytical Chemistry Fresenius' Journal of Analytical Chemistry Fresenius Zeitschrift f�r Analytische Chemie.  409:439-451. 2017
    2015 The glycosylation-dependent interaction of perlecan core protein with LDL: implications for atherosclerosis.Journal of Lipid Research.  56:266-276. 2015
    2014 Structural characterization by multistage mass spectrometry (MSn) of human milk glycans recognized by human rotaviruses.Molecular and Cellular Proteomics.  13:2961-2974. 2014
    2013 Toward a platform for comprehensive glycan sequencing.Molecular and Cellular Proteomics.  12:866-873. 2013
    2010 Rapid de-O-glycosylation concomitant with peptide labeling using microwave radiation and an alkyl amine base.Analytical Chemistry Industrial and Engineering Chemistry Analytical Edition.  82:2421-2425. 2010
    2009 O-glycosylation pattern of CD24 from mouse brain.Biological Chemistry.  390:627-645. 2009
    2009 The high mannose glycans from bovine ribonuclease B isomer characterization by ion trap MS.Journal of the American Society for Mass Spectrometry.  20:539-556. 2009
    2009 Glycomic analysis of N-linked carbohydrate epitopes from CD24 of mouse brain.Journal of Proteome Research.  8:567-582. 2009
    2007 Comparison of the methods for profiling glycoprotein glycans--HUPO Human Disease Glycomics/Proteome Initiative multi-institutional study.Glycobiology.  17:411-422. 2007
    2005 Congruent strategies for carbohydrate sequencing. 1. Mining structural details by MSn.Analytical Chemistry Industrial and Engineering Chemistry Analytical Edition.  77:6250-6262. 2005
    2005 Congruent strategies for carbohydrate sequencing. 3. OSCAR: an algorithm for assigning oligosaccharide topology from MSn data.Analytical Chemistry Industrial and Engineering Chemistry Analytical Edition.  77:6271-6279. 2005
    2005 Structural characterization of the major extrapallial fluid protein of the mollusc Mytilus edulis: implications for function.Biochemistry.  44:10720-10731. 2005
    2005 Nontypeable Haemophilus influenzae-binding gangliosides of human respiratory (HEp-2) cells have a requisite lacto/neolacto core structure.FEMS Immunology and Medical Microbiology.  45:171-182. 2005
    2003 Antigens in tea-beverage prime human Vgamma 2Vdelta 2 T cells in vitro and in vivo for memory and nonmemory antibacterial cytokine responses.Proceedings of the National Academy of Sciences of the United States of America.  100:6009-6014. 2003
    2002 Neisseria gonorrhoeae strain PID2 simultaneously expresses six chemically related lipooligosaccharide structures.Glycobiology.  12:523-533. 2002
    2001 Structural and immunochemical characterization of the lipooligosaccharides expressed by Neisseria subflava 44.Journal of Bacteriology.  183:942-950. 2001
    1995 Structure and function of laminin 1 glycans; glycan profiling.Glycobiology.  5:157-158. 1995
    1994 Synthesis of glycerophosphorylated cyclic beta-(1,2)-glucans by Rhizobium meliloti ndv mutants.Journal of Bacteriology.  176:1047-1051. 1994
    1990 Cell-associated oligosaccharides of Bradyrhizobium spp.Journal of Bacteriology.  172:136-142. 1990
    1989 Modification of sialyl residues of glycoconjugates by reductive amination. Characterization of the modified sialic acids.Carbohydrate Research.  186:255-265. 1989
    1987 Cyclic glucans produced by Agrobacterium tumefaciens are substituted with sn-1-phosphoglycerol residues.Biochimica et Biophysica Acta - General Subjects.  901:112-118. 1987
    1987 The high mannose oligosaccharides of Dictyostelium discoideum glycoproteins contain a novel intersecting N-acetylglucosamine residue.Journal of Biological Chemistry.  262:4521-4527. 1987
    1986 Characterization of O-glycosidically linked oligosaccharides of rat erythrocyte membrane sialoglycoproteins.Biochemistry.  25:8017-8024. 1986
    1986 Osmotic adaptation by gram-negative bacteria: possible role for periplasmic oligosaccharides.Science The Scientific monthly.  231:48-51. 1986
    1980 Oligosaccharides of human bronchial glycoproteins. Neutral di- and trisaccharides isolated from a patient suffering from chronic bronchitis.Journal of Biological Chemistry.  255:4595-4598. 1980
    1979 Chemical structure of epiglycanin, the major glycoprotein of the TA3-Ha ascites cell. The carbohydrate chains.Journal of Biological Chemistry.  254:12153-12159. 1979
    1979 Structural studies of the membrane-derived oligosaccharides of Escherichia coli.Journal of Biological Chemistry.  254:10135-10138. 1979
    1979 N-nitrosamines in the rubber and tire industry.Science The Scientific monthly.  205:1262-1264. 1979
    1979 CHEMICAL-STRUCTURE OF EPIGLYCANIN, THE MAJOR GLYCOPROTEIN OF THE TA3-HA ASCITES CELL - CARBOHYDRATE CHAINSJournal of Biological Chemistry.  254:2153-2159. 1979
    1979 STRUCTURAL STUDIES OF THE MEMBRANE-DERIVED OLIGOSACCHARIDES OF ESCHERICHIA-COLIJournal of Biological Chemistry.  254:135-138. 1979
    1973 2-Amino-2-deoxyhexuronic acid: a constituent of Shigella sonnei phase I lipopolysaccharide.FEBS Journal European Journal of Biochemistry.  36:160-166. 1973
    1971 The olfactory and auditory mediated sex attraction in Achroia grisella (Fabr.).Die Naturwissenschaften.  58:265-266. 1971
    1970 Identification of the drug Darvon and its metabolites in the urine of a comatose patient using a gas chromatograph-mass spectrometer-computer system.Experientia.  26:714-717. 1970

    Conference Proceeding

    Year Title
    2015 GAG Sequencing, Aided by Sulfate Site Isotope Exchange and MSnGlycobiology. 1272-1272. 2015
    2014 Core Fucosylation: A Key To The Activity of 1918 H1N1 NeuraminidaseGlycobiology. 1146-1146. 2014
    2014 The glycosylation-dependent interaction of perlecan core protein with LDL: implications for atherosclerosisGlycobiology. 1194-1194. 2014
    2013 The Human Platelet Glycome and Its Variations Among Healthy Volunteers and StorageBlood. 2013
    2013 Functional Glycan Epitopes: Sequential Mass Spectrometry and Library MatchingGlycobiology. 1359-1360. 2013
    2012 Glycosylation-Dependent Lipoprotein Binding of Low-Density Lipoprotein Receptor-Like Domain of Perlecan and Implications for AtherosclerosisCirculation. 2012
    2005 Aberrant protein N-glycosylation in Caenorhabditis elegans GnT-I triple knockout wormsGlycobiology. 1218-1218. 2005
    2005 Computational model of cytokine receptor regulation by hexosamine and golgi N-glycosylation pathwaysGlycobiology. 1235-1236. 2005
    2005 Discrimination of isomeric/isobaric glycosphingolipid glycan structures using ion trap MS" in combination with glycan fragment library and decomposition pathway constraint strategiesGlycobiology. 1209-1209. 2005
    2004 Computational model of cytokine receptor regulation by hexosamine and golgi N-glycosylation pathwaysGlycobiology. 1110-1110. 2004
    2004 De novo MSn sequencing with Contiguous Glycan Segments (CGS)Glycobiology. 1191-1191. 2004
    2004 Glycan characterization using a MSn fragment fingerprint libraryGlycobiology. 1192-1192. 2004
    2004 Structural diversity of C-elegans glycomeGlycobiology. 1125-1125. 2004
    2003 Abundant and unusual N-linked glycans from the eukaryote, C. elegansGlycobiology. 899-900. 2003
    2003 Composition to sequence: A novel computational approach to support MSn carbohydrate sequencingGlycobiology. 848-848. 2003
    2003 Glycan disassembly by MSn: Linkage, branching and monomer identificationGlycobiology. 848-848. 2003
    2003 Regulation of N-glycosylation and cytokine signaling by the hexamine pathwayGlycobiology. 867-868. 2003
    2003 Specific, non-reductive O-linked deglycosylation coupled with enhanced MALDI-MS detectionGlycobiology. 849-849. 2003
    2003 Studies on Caenorhabditis elegans with a null mutation in UDP-GlcNAc : alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I (GnT I): synthesis of paucimannose N-glycans requires both GnT I and a specific membrane-bound beta-N-acetylglucosaminidaseFASEB Journal. A176-A176. 2003
    2002 HTP proteome-glycome analysis in Caenorhabditis elegansGlycobiology. 650-650. 2002
    2002 Trapping a glycome profile. The "GlycoTrap"Glycobiology. 677-678. 2002
    2000 Detailed structural analysis of milk oligosaccharides by Paul trap MSnGlycobiology. 1128-1128. 2000
    2000 Glycosylation structure-function relationships with Caenorhabditis elegans; the proteome and glycome.Glycobiology. 1128-1129. 2000
    2000 Neisseria OS: Introduction of a protecting reducing-end-tagGlycobiology. 1126-1126. 2000
    2000 Structural characterization of n-linked glycan from biomineralization matrix protein of Mytilus edulisGlycobiology. 1119-1119. 2000
    1998 Ion trapping MS and understanding carbohydrate structural detailGlycobiology. 1112-1112. 1998
    1998 T cell recognition of self and foreign hexose phospholipids presented by CD1c.Arthritis and Rheumatism Arthritis care and research : the official journal of the Arthritis Health Professions Association. S350-S350. 1998
    1996 Cellular immunity mediated by CD1 restricted, non peptide antigen presentation: Mass spectral analyses of antigens from Mycobacterium phleiGlycobiology. 1220-1220. 1996
    1985 OSMOTIC REGULATION OF CYCLIC BETA-1-]2 GLUCAN BIOSYNTHESIS IN AGROBACTERIUM-TUMEFACIENSFASEB Journal. 1437-1437. 1985
    1984 HPLC SEPARATION, IDENTIFICATION AND ISOLATION OF FLUORESCENTLY LABELED OLIGOSACCHARIDES FOR STRUCTURAL STUDIESACS National Meeting Book of Abstracts. 14-CARB. 1984
    1978 ISOLATION AND PARTIAL IDENTIFICATION OF SEVERAL NEW POLAR METABOLITES OF ALDOSTERONE SYNTHESIZED IN LIVER OF MALE RATSJournal of Steroid Biochemistry and Molecular Biology. 828-828. 1978
    1975 ISOLATION AND CHARACTERIZATION OF ELLIPTICINE METABOLITES OBTAINED FROM RAT BILEPROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH. 135-135. 1975

    Principal Investigator On

  • Shaping MSn Technology for High Impact Glycan Sequencing  awarded by US DHHS, National Institutes of Health 2017 - 2019
  • Facile Chemical Methods for Reverse Synthesis and Large Scale Production of Biomedically Relevant Glycan Libraries  awarded by Emory University 2015 - 2019
  • Programs of Excellence in Glycosciences (PO1)  awarded by Brigham and Women's Hospital 2017 - 2018
  • Programs of Excellence in Glycosciences (PO1)  awarded by Brigham and Women's Hospital 2016 - 2017
  • Programs of Excellence in Glycosciences (PO1)  awarded by Brigham and Women's Hospital 2015 - 2016
  • Programs of Excellence in Glycosciences (PO1)  awarded by Brigham and Women's Hospital 2014 - 2015
  • Programs of Excellence in Glycosciences (PO1)  awarded by Brigham and Women's Hospital 2013 - 2014
  • Programs of Excellence in Glycosciences (PO1)  awarded by Brigham and Women's Hospital 2012 - 2013
  • Programs of Excellence in Glycosciences (PO1)  awarded by Brigham and Women's Hospital 2011 - 2012
  • Mass Spectral Sequencing of Carbohydrates  awarded by US DHHS, National Institutes of Health 2006 - 2011
  • Identify Glycosylation Patterns of Immune Cells and Properties of the Infecting Organism  awarded by Women and Infants Hospital 2010
  • Characterization of Belatacept N- and O- Linked Glycosylation  awarded by Bristol-Myers Squibb 2007 - 2009
  • 2nd Annual Glucoconjugate Analysis Workshop  awarded by US DHHS, National Institutes of Health 2007 - 2008
  • Glyconjugate Analysis Workshop  awarded by US DHHS, National Institutes of Health 2006 - 2007
  • CD1 Acquisition of BCR/Fc Receptor Targeted Ligand  awarded by Dartmouth College 2005 - 2006
  • Center for Structural Biology  awarded by US DHHS, National Institutes of Health 2001 - 2006
  • CD1 Acquisition of BCR/Fc Receptor Targeted Ligand  awarded by Dartmouth College 2004 - 2005
  • CD1 Acquisition of BCR/Fc Receptor Targeted Ligand  awarded by Dartmouth College 2003 - 2004
  • CD1 Acquisition of BCR/Fc Receptor Targeted Ligand  awarded by Dartmouth College 2002 - 2003
  • CD1 Acquisition of BCR/Fc Receptor Targeted Ligand  awarded by Dartmouth College 2000 - 2002
  • Teaching Activities

  • Rsrch Exp/MCBS Taught course 2016
  • Doctoral Research Taught course 2015
  • Rsrch Exp/MCBS Taught course 2015
  • Rsrch Exp/MCBS Taught course 2014
  • Education And Training

  • B.S. Chemistry, University of New Hampshire
  • M.S. Biochemistry, University of New Hampshire
  • Ph.D. Biochemistry, University of Vermont
  • Full Name

  • Vernon Reinhold