Structural studies of the membrane-derived oligosaccharides of Escherichia coli.

Academic Article

Abstract

  • Membrane-derived oligosaccharides are a novel class of glucose-containing oligosaccharides found in the cell envelope of Escherichia coli and other Gram-negative organisms (Schulman, H., AND Kennedy, E.P. (1979) J. Bacteriol. 137, 686-688). Previous work has shown that these oligosaccharides contain sn-1-glycero-P and smaller amounts of phosphoethanolamine, derived from membrane phospholipids, attached to position 6 of certain of the glucose residues. The structure of the parent oligosaccharides (obtained by reduction with borohydride followed by alkaline hydrolysis) has now been studied. The oligosaccharide was permethylated, followed by hydrolysis and conversion of the products to methylated glucitol acetates, which were then analyzed and identified by gas-liquid chromatography and mass spectrometry. The membrane oligosaccharides contain 10 to 12 D-glucopyranoside residues/mol, linked solely by 1 yields 2 and 1 yields 6 bonds. They are highly branched structures, with four nonreducing termini per mol. Glucose units at the branch points are doubly substituted at positions 2 and 6. The low specific rotation of the oligosaccharide (+8.3 degrees) indicates that the glycosidic bonds are predominantly or entirely beta.
  • Authors

  • Schneider, JE
  • Reinhold, Vernon
  • Rumley, MK
  • Kennedy, EP
  • Status

    Publication Date

  • October 25, 1979
  • Published In

    Keywords

  • Carbohydrate Conformation
  • Cell Membrane
  • Chromatography, Gas
  • Escherichia coli
  • Glycosides
  • L-Iditol 2-Dehydrogenase
  • Oligosaccharides
  • Pubmed Id

  • 385596
  • Start Page

  • 10135
  • End Page

  • 10138
  • Volume

  • 254
  • Issue

  • 20