The high mannose oligosaccharides of Dictyostelium discoideum glycoproteins contain a novel intersecting N-acetylglucosamine residue.

Academic Article


  • Previous studies have shown that the majority of the asparagine-linked high mannose oligosaccharide units present on the lysosomal enzymes of the slime mold Dictyostelium discoideum contain mannose 6-sulfate and mannose 6-phosphate residues, the latter being diesterified to methyl groups. Using a variety of techniques, including NMR spectroscopy and fast atom bombardment mass spectrometry, we have found that many of these oligosaccharides also contain an N-acetylglucosamine residue linked beta 1-4 to the mannose residue linked alpha 1-6 to the beta-linked core mannose. (Formula: see text) As far as we are aware, this is the first description of an intersecting N-acetylglucosamine residue in this position on a high mannose oligosaccharide.
  • Authors

  • Couso, R
  • van Halbeek, H
  • Reinhold, Vernon
  • Kornfeld, S
  • Status

    Publication Date

  • April 5, 1987
  • Published In


  • Acetylglucosamine
  • Carbohydrate Sequence
  • Dictyostelium
  • Fungal Proteins
  • Glycoproteins
  • Magnetic Resonance Spectroscopy
  • Mannose
  • Mass Spectrometry
  • Oligosaccharides
  • Pubmed Id

  • 3558352
  • Start Page

  • 4521
  • End Page

  • 4527
  • Volume

  • 262
  • Issue

  • 10