Changes to landscapes for agricultural activities often result in reduction and fragmentation of forested habitat. Land conversion for cattle ranching in south-central Florida has resulted in increases in pasture land interspersed with remnant patches of hardwood hammock. To examine the importance of these hammocks to anurans, we sampled 78 seasonally inundated wetlands to examine the relative importance of proximity of hardwood hammock patches (>20 ha) and wetland characteristics and used generalized linear models to determine which factors had a significant effect on larval anuran species richness or abundance. Species richness was significantly influenced by pH, conductivity, and water depth. Proximity to hammock did not influence species richness; however, assemblage composition differed between wetlands near hammocks and wetlands surrounded by pasture. Barking treefrogs ( Hyla gratiosa LeConte, 1856), pine woods treefrogs ( Hyla femoralis Bosc in Daudin, 1800), and oak toads ( Bufo quercicus Holbrook, 1840) bred only in wetlands within 20, 50, and 200 m of hammocks, respectively. Factors influencing tadpole abundances were species-specific. Retention of seasonally inundated wetlands proximal to large hammocks on ranchlands can provide important habitat for supporting a diverse assemblage of anurans.