Cytoplasmic free-Ca2+ level rises with repellents and falls with attractants in Escherichia coli chemotaxis.

Academic Article


  • Cytoplasmic free-Ca2+ levels in Escherichia coli were measured by use of the fluorescent Ca(2+)-indicator dye fura-2. Chemotactically wild-type E. coli regulated cytoplasmic free Ca2+ at approximately 100 nM when no stimuli were encountered, but changes in bacterial behavior correlated with changes in cytoplasmic free-Ca2+ concentration. For chemotactically wild-type E. coli, addition of a repellent resulted in cells tumbling and a transient increase in cytoplasmic free-Ca2+ levels. Conversely, addition of an attractant to wild-type cells caused running and produced a transient decrease in cytoplasmic free-Ca2+ levels. Studies with mutant strains showed that the chemoreceptors were required for the observed changes in cytoplasmic free-Ca2+ levels in response to chemical stimuli.
  • Authors

  • Tisa, Louis
  • Adler, J
  • Status

    Publication Date

  • November 7, 1995
  • Keywords

  • Amino Acids
  • Calcium
  • Chemotaxis
  • Cytoplasm
  • Electroporation
  • Escherichia coli
  • Fura-2
  • Mutation
  • Receptors, Cell Surface
  • Spectrometry, Fluorescence
  • Digital Object Identifier (doi)

    Start Page

  • 10777
  • End Page

  • 10781
  • Volume

  • 92
  • Issue

  • 23