Calcium transport by Frankia sp. strain EAN1pec.

Academic Article


  • When incubated at 25 degrees C, N2-grown cells of Frankia strain EAN1pec actively accumulated calcium, while NH4Cl-grown cells did not accumulate calcium. When incubated at 0 degrees C, both N2-grown and NH4Cl-grown cells did not actively accumulate calcium. Inhibitors of respiration inhibited calcium accumulation by N2-grown cells at 25 degrees C. Isolated vesicles also accumulated calcium in an energy- and temperature-dependent manner. Two lines of evidence show that Frankia strain EAN1pec has an active calcium extrusion mechanism. First, NH4Cl-grown cells incubated under deenergizing conditions accumulated calcium. Second, calcium efflux from calcium-loaded cells required an energy source and was blocked by inhibitors. The results of this study indicate that Frankia strain EAN1pec has two systems for calcium transport: a calcium extrusion system and a developmentally regulated calcium uptake system.
  • Authors


    Publication Date

  • July 1998
  • Keywords

  • Actinomycetales
  • Ammonium Chloride
  • Calcium
  • Calcium Radioisotopes
  • Temperature
  • Time Factors
  • Digital Object Identifier (doi)

    Pubmed Id

  • 9625783
  • Start Page

  • 12
  • End Page

  • 16
  • Volume

  • 37
  • Issue

  • 1