Renal organic anion secretion: evidence for dopaminergic and adrenergic regulation.

Academic Article


  • To examine possible regulatory control of renal proximal tubule organic anion secretion, winter flounder (Pleuronectes americanus) proximal tubule primary cultures were mounted in Ussing chambers. Unidirectional fluxes of [2,4-(14)C]dichlorophenoxyacetic acid were determined under short-circuited conditions. Phorbol 12-myristate 13-acetate (1 microM) caused a significant (P < 0.01) inhibition of net 2,4-dichlorophenoxyacetic acid secretion. Preincubation with staurosporine (1 microM) blocked the phorbol 12-myristate 13-acetate-induced decrease in secretion. Neither forskolin (10 microM) nor W-7 (20 microM) had any effect on net transport. Elevation of intracellular calcium activity with either A-23187 or thapsigargin produced a slight, transient decrease in transport. Addition of dopamine (1 microM) to the peritubular side, but not the luminal side, caused a significant (P < 0.01) decrease in net secretion. Both the alpha-adrenergic agonist oxymetazoline (10 microM) and depletion of intracellular Na+ transiently, but significantly (P < 0.05), increased net transport. The data indicate that renal organic anion excretion may be regulated through dopaminergic inhibition and alpha-adrenergic stimulation of net transepithelial secretion.
  • Authors

  • Halpin, Patricia
  • Renfro, JL
  • Status

    Publication Date

  • November 1996
  • Keywords

  • 2,4-Dichlorophenoxyacetic Acid
  • Adrenergic Agents
  • Adrenergic alpha-Agonists
  • Animals
  • Anions
  • Calcium
  • Dopamine
  • Enzyme Activation
  • Flounder
  • Intracellular Membranes
  • Kidney Tubules, Proximal
  • Oxymetazoline
  • Probenecid
  • Protein Kinases
  • Digital Object Identifier (doi)

    Pubmed Id

  • 8945976
  • Start Page

  • R1372
  • End Page

  • R1379
  • Volume

  • 271
  • Issue

  • 5 Pt 2