Direct determination of macromolecular charge by equilibrium electrophoresis.

Academic Article


  • Charge is a fundamental property of macromolecules in solution. However, estimation of the apparent charge on polyions has confounded science for decades. Presented here is a general method to determine directly the apparent charge on a polyion, regardless of its size or shape. This new method uses equilibrium electrophoresis, a procedure in which opposing solute flows from electrophoresis and from diffusion balance everywhere as the system reaches a steady-state distribution. The method uses only small quantities of materials, is nondestructive, and requires only simple, inexpensive instrumentation. Here we describe a prototype apparatus, demonstrate the phenomenon, and present experimental examples of the procedure.
  • Authors

  • Laue, TM
  • Hazard, AL
  • Ridgeway, Theresa
  • Yphantis, DA
  • Status

    Publication Date

  • November 1, 1989
  • Published In


  • Chemical Phenomena
  • Chemistry
  • Cytochrome c Group
  • Electrophoresis
  • Hydrogen-Ion Concentration
  • Least-Squares Analysis
  • Macromolecular Substances
  • Digital Object Identifier (doi)

    Pubmed Id

  • 2558592
  • Start Page

  • 377
  • End Page

  • 382
  • Volume

  • 182
  • Issue

  • 2