Delayed or persistent episodes of subclinical hypocalcemia (SCH) have been associated with reduced milk production and feed intake and they put cows at risk for additional disease development. However, little is known about the effect of delayed or persistent SCH on reproductive outcomes. The objectives of our study were to describe the association between SCH status at 4 d in milk (DIM) with the odds of pregnancy to first service and time to pregnancy through 150 DIM. Data were collected from multiparous Holstein cows (n = 697) from 4 farms in New York State that did not receive supplemental Ca during the first 5 DIM. Cows were classified into 1 of 2 SCH groups based on blood total Ca (tCa) at 4 DIM: normocalcemic (NC; n = 515) if tCa >2.2 mmol/L at 4 DIM or SCH (n = 182) if tCa ≤2.2 mmol/L at 4 DIM. We created a multivariable logistic regression model to assess the association between SCH group and pregnancy to first service and a time-to-event analysis to evaluate the effect of SCH group on the hazard of pregnancy by 150 DIM. Cows that were SCH at 4 DIM had lower odds of pregnancy to first service [odds ratio = 0.75, 95% confidence interval (CI) = 0.61 to 0.93, incidence = 18.1%] compared with their NC counterparts (incidence = 27.4%). Similarly, SCH cows tended to have a lower hazard of pregnancy by 150 DIM (hazard ratio = 0.82, 95% CI = 0.67 to 1.01, incidence = 65.4%) than NC cows (incidence = 70.7%). Our results indicate that delayed or prolonged reductions in blood Ca in the early postpartum period, resulting in SCH at 4 DIM, were associated with reduced reproductive outcomes in multiparous cows. Our findings, in conjunction with previous reports of the negative associations of delayed or persistent SCH on health and production, suggest that reductions in blood Ca beyond the first day of lactation might be indicative of maladaptation to lactation, and the ramifications can persist beyond the immediate postpartum period.
