We have had significant success in building cyclodextrin mediated sensors. We initially started with a gold surface, being able to detect analyte up to three times post modification [1]. Afterwards, we improved the stability and reusability by moving to a phenyl-modified carbon electrode via diazonium salt chemistry [2]. With his new surface, we were able to detect cortisol up to 10 different times with high accuracy, repeatability, and sensitivity in buffer, urine, and artificial saliva. We were limited by manually manipulating the electrode; therefore, we were not able to test the electrode for greater than 10 measurements. Within this poster, we intend to present our ideas and progress towards developing an automated detection system that can be preprogrammed to clean, reset, and test the cyclodextrin-mediated surface with target analytes. Further, we will share our various target analytes and our strategy to expand our sensor surface to include multiple cyclodextrin derivatives.
Research supported by New Hampshire- INBRE through an Institutional Development Award (IDeA), P20GM103506, from the National Institute of General Medical Sciences of the NIH.
[1] Z. Panahi, M. A. Merrill, and J. M. Halpern, “Reusable Cyclodextrin-Based Electrochemical Platform for Detection of trans -Resveratrol,” ACS Appl. Polym. Mater., vol. 2, no. 11, pp. 5086–5093, Nov. 2020.
[2] Z. Panahi, T. Ren, and J. M. Halpern, “Nanostructured Cyclodextrin-Mediated Surface for Capacitive Determination of Cortisol in Multiple Biofluids,” ACS Appl. Mater. Interfaces, Aug. 2022.
