[Cloning expression of a novel human protein-disulfide isomerase like cDNA].

Academic Article


  • Using the large scale sequencing, a novel human cDNA of 1,645 bp was screened from the cDNA library of human fetal brain. The cDNA contains an ORF encoding a 296-aa protein with a calculated molecular weight of 34.0 KD. Compared with that of current sequence databases, the putative protein was found to have 36% homology with protein-disulfide isomerase (PDI). So this cDNA was named PDI like (PDI-L) gene. Multiple tissue Northern blot analysis shows that PDI-L cDNA is expressed in heart, brain, liver, kidneys and so on. The ORF fragment of PDI-L cDNA was inserted into reconstructed pBV220 expression vehicle and its predicted expressive protein was obtained.
  • Authors

  • Huang, Jin
  • Yang, Lei
  • Zhang, Yulan
  • Chen, Xuanmao
  • Ying, Kang
  • Xie, Yi
  • Status

    Publication Date

  • September 2002
  • Keywords

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Gene Library
  • Humans
  • Protein Disulfide-Isomerases
  • Pubmed Id

  • 12557522
  • Start Page

  • 459
  • End Page

  • 462
  • Volume

  • 19
  • Issue

  • 3