Indirect fluorescence detection of amino sugars with the use of copper complexes of tryptophan and its analogues following high-performance liquid chromatographic separation.

Academic Article

Abstract

  • A simple, indirect fluorescence detection method has been developed for detecting specific mono-amino sugars (D-glucosamine, D-galactosamine, D-mannosamine) following chromatographic separation. The eluting amino sugars release L-tryptophan (L-Trp) from a copper-tryptophan complex which is introduced postcolumn. Analyte detection is based on measuring the increase in L-Trp fluorescence, which is quenched when complexed with copper. Two tryptophan analogues, 5-hydroxy-L-tryptophan (5-HTP) and DL-5-methoxytryptophan (5-MTP), were also evaluated as postcolumn reagents. 5-MTP was found to be a suitable alternative to L-Trp for the detection of these mono-amino sugars. Detection limits for D-glucosamine, D-galactosamine, and D-mannosamine are in the range of 0.15-0.30 nmol injected.
  • Authors

  • Shen, Xiaoxuan
  • Yang, Min
  • Tomellini, Sterling
  • Status

    Publication Date

  • April 29, 2005
  • Keywords

  • Amino Sugars
  • Chromatography, High Pressure Liquid
  • Copper
  • Hydrogen-Ion Concentration
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence
  • Tryptophan
  • Digital Object Identifier (doi)

    Pubmed Id

  • 15887498
  • Start Page

  • 273
  • End Page

  • 277
  • Volume

  • 1072
  • Issue

  • 2