Capture and storage of plant genomic DNA on a readily available cellulose matrix.

Academic Article

Abstract

  • Whatman FTA® Cards are a fast and efficient method for capturing and storing nucleic acids but can be cost-prohibitive for large numbers of samples. We developed a method that substitutes a readily-available cellulose matrix and homemade washing buffer for commercial FTA® Cards and FTA® Purification Reagent. This method is suitable for long-term storage of DNA from many plant species prior to PCR.
  • Authors

  • Thompson, Megan M
  • Hrabak, Estelle
  • Status

    Publication Date

  • November 2018
  • Published In

  • BioTechniques  Journal
  • Keywords

  • Buffers
  • Cellulose
  • DNA
  • DNA, Plant
  • FTA purification reagent
  • FTA® Cards
  • Genes, Plant
  • Genome, Plant
  • Genomics
  • Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
  • Indicators and Reagents
  • PCR
  • Plant Proteins
  • Plants
  • Polymerase Chain Reaction
  • polymerase chain reaction
  • Digital Object Identifier (doi)

    Pubmed Id

  • 30394128
  • Start Page

  • 285
  • End Page

  • 287
  • Volume

  • 65
  • Issue

  • 5