Oxytocin-specific RNA, oxytocin and progesterone concentrations in corpora lutea of heifers treated with oxytocin.

Academic Article

Abstract

  • Sixteen virgin Holstein heifers were used to study the role of oxytocin in luteal function. Oxytocin (100 i.u.) was administered on Days 2-5 (Exp. 1) or Days 2-6 (Exp. 2), and the corpora lutea were collected and weighed on Day 6 or Day 8, respectively. Plasma progesterone values and tissue concentrations of progesterone and oxytocin were determined by radioimmunoassay. In addition, the tissue level of the oxytocin-specific transcript was measured by dot-blot hybridization. Expression of the oxytocin-specific transcript was not affected by oxytocin treatment in either experiment. In contrast, plasma progesterone values and tissue progesterone and oxytocin concentrations were all decreased after 5 days of oxytocin treatment (Exp. 2), but not after 4 days (Exp. 1). Since oxytocin concentrations were reduced while the level of its transcript remained unaffected, oxytocin injections might be influencing either peptide processing or release.
  • Authors

  • Tsang, Paul
  • Walton, JS
  • Hansel, W
  • Status

    Publication Date

  • May 1990
  • Keywords

  • Animals
  • Cattle
  • Corpus Luteum
  • DNA Probes
  • Female
  • Organ Size
  • Oxytocin
  • Progesterone
  • RNA
  • Radioimmunoassay
  • Transcription, Genetic
  • Digital Object Identifier (doi)

    Pubmed Id

  • 1695681
  • Start Page

  • 77
  • End Page

  • 84
  • Volume

  • 89
  • Issue

  • 1