Proper Controls to Electrochemically Evaluate Carotenoids using β-Cyclodextrin Modified Surfaces.

Academic Article


  • We initially tested the electrochemical activity of beta-carotene and lutein at unmodified glassy carbon electrodes. We found good sensitivity (1 nA/μM) at high, micromolar concentrations, but serum levels are at nanomolar concentrations. To enhance the electrochemical activity, we modified the sensor surface with β-cyclodextrin, which has a hydrophobic core. Our goal was that the beta-carotene will be attracted to the β-cyclodextrin core, increasing surface interaction and sensitivity. Instead we saw a decrease in electrochemical activity. Further investigation with a methylene blue mediator indicated two results. First, it is unlikely the beta-carotene strongly interacts with the β-cyclodextrin surface. And, second, the presence of a co-solvent or surfactant can greatly disrupt the surface β-cyclodextrin activity.
  • Authors

  • Thompson, Garrett
  • Marnoto, Sabrina
  • Halpern, Jeffrey
  • Status

    Publication Date

  • October 2017
  • Published In

  • ECS Transactions  Journal
  • Digital Object Identifier (doi)

    Start Page

  • 1177
  • End Page

  • 1187
  • Volume

  • 80
  • Issue

  • 10