A Low-Cost Inkjet-Printed Aptamer-Based Electrochemical Biosensor for the Selective Detection of Lysozyme.

Academic Article

Abstract

  • Recently, inkjet-printing has gained increased popularity in applications such as flexible electronics and disposable sensors, as well as in wearable sensors because of its multifarious advantages. This work presents a novel, low-cost immobilization technique using inkjet-printing for the development of an aptamer-based biosensor for the detection of lysozyme, an important biomarker in various disease diagnosis. The strong affinity between the carbon nanotube (CNT) and the single-stranded DNA is exploited to immobilize the aptamers onto the working electrode by printing the ink containing the dispersion of CNT-aptamer complex. The inkjet-printing method enables aptamer density control, as well as high resolution patternability. Our developed sensor shows a detection limit of 90 ng/mL with high target selectivity against other proteins. The sensor also demonstrates a shelf-life for a reasonable period. This technology has potential for applications in developing low-cost point-of-care diagnostic testing kits for home healthcare.
  • Authors

  • Khan, Niazul Islam
  • Maddaus, Alec G
  • Song, Edward
  • Publication Date

  • January 15, 2018
  • Published In

  • Biosensors  Journal
  • Keywords

  • Animals
  • Aptamers, Nucleotide
  • Biosensing Techniques
  • Chickens
  • Electrochemical Techniques
  • Electrodes
  • Muramidase
  • Printing
  • aptamer
  • biosensor
  • electrochemical impedance spectroscopy
  • inkjet printing
  • lysozyme
  • point-of-care
  • Digital Object Identifier (doi)

    Start Page

  • 7
  • End Page

  • 7
  • Volume

  • 8
  • Issue

  • 1