Synthesis of glycerophosphorylated cyclic beta-(1,2)-glucans by Rhizobium meliloti ndv mutants.

Academic Article

Abstract

  • The periplasmic cyclic beta-(1,2)-glucans of Rhizobium spp. are believed to provide functions during hypoosmotic adaptation and legume nodulation. In Rhizobium meliloti, cyclic beta-(1,2)-glucans are synthesized at highest levels when cells are grown at low osmolarity, and a considerable fraction (> or = 35%) of these glucans may become substituted with phosphoglycerol moieties. Thus far, two chromosomally encoded proteins, NdvA and NdvB, have been shown to function during cyclic beta-(1,2)-glucan biosynthesis; however, the precise roles for these proteins remain unclear. In the present study, we show that R. meliloti mutants lacking up to one-third of the downstream region of ndvB synthesize cyclic beta-(1,2)-glucans similar to those produced by wild-type cells with respect to size and phosphoglycerol substituent profile. In contrast, no phosphoglycerol substituents were detected on the cyclic beta-(1,2)-glucans synthesized by an R. meliloti ndvA mutant.
  • Authors

  • Breedveld, MW
  • Yoo, JS
  • Reinhold, Vernon
  • Miller, KJ
  • Status

    Publication Date

  • February 1994
  • Published In

    Keywords

  • ATP-Binding Cassette Transporters
  • Anions
  • Bacterial Proteins
  • Ferredoxins
  • Glucans
  • Glycerophosphates
  • Magnetic Resonance Spectroscopy
  • Mutation
  • Rhizobium
  • Sinorhizobium meliloti
  • Species Specificity
  • Spectrometry, Mass, Fast Atom Bombardment
  • beta-Glucans
  • Digital Object Identifier (doi)

    Start Page

  • 1047
  • End Page

  • 1051
  • Volume

  • 176
  • Issue

  • 4