Cyclic glucans produced by Agrobacterium tumefaciens are substituted with sn-1-phosphoglycerol residues.

Academic Article


  • In a previous study (Miller, K.J., Kennedy, E.P. and Reinhold, V.N. (1986) Science 231, 48-51) it was reported that the biosynthesis of periplasmic cyclic beta-1,2-glucans by Agrobacterium tumefaciens is strictly osmoregulated in a pattern closely similar to that found for the membrane-derived oligosaccharides of Escherichia coli (Kennedy, E.P. (1982) Proc. Natl. Acad. Sci. USA 79, 1092-1095). In addition to the well-characterized neutral cyclic glucan, the periplasmic glucans were found to contain an anionic component not previously reported. Biosynthesis of the anionic component is osmotically regulated in a manner indistinguishable from that of the neutral cyclic beta-1,2-glucan. We now find that the anionic component consists of cyclic beta-1,2-glucans substituted with one or more sn-1-phosphoglycerol residues. The presence of sn-1-phosphoglycerol residues represents an additional, striking similarity to the membrane-derived oligosaccharides of E. coli.
  • Authors

  • Miller, KJ
  • Reinhold, Vernon
  • Weissborn, AC
  • Kennedy, EP
  • Status

    Publication Date

  • July 10, 1987
  • Keywords

  • Anions
  • Escherichia coli
  • Glucans
  • Glucose
  • Glycerophosphates
  • Mass Spectrometry
  • Oligosaccharides
  • Rhizobium
  • Digital Object Identifier (doi)

    Pubmed Id

  • 3297148
  • Start Page

  • 112
  • End Page

  • 118
  • Volume

  • 901
  • Issue

  • 1