Drug targeting Mycobacterium tuberculosis cell wall synthesis: development of a microtiter plate-based screen for UDP-galactopyranose mutase and identification of an inhibitor from a uridine-based library.

Academic Article

Abstract

  • A microtiter plate assay for UDP-galactopyranose mutase, an essential cell wall biosynthetic enzyme of Mycobacterium tuberculosis, was developed. The assay is based on the release of tritiated formaldehyde from UDP-galactofuranose but not UDP-galactopyranose by periodate and was used to identify a uridine-based enzyme inhibitor from a chemical library.

    • Authors

  • Scherman, Michael S
  • Winans, Katharine
  • Stern, Richard J
  • Jones, Victoria
  • Bertozzi, Carolyn R
  • McNeil, Michael R

    • Status

    Publication Date

  • January 2003

    • Has Subject Area

    Published In

    Keywords

  • Cell Wall
  • Enzyme Inhibitors
  • Intramolecular Transferases
  • Mycobacterium tuberculosis
  • Structure-Activity Relationship

    • Digital Object Identifier (doi)

    Start Page

  • 378

    • End Page

  • 382

    • Volume

  • 47

    • Issue

  • 1