Shoot regeneration from stem and leaf callus of Eucalyptus tereticornis.

Academic Article

Abstract

  • Adventitious shoots were obtained from leaf and stem callus of Eucalyptus tereticornis SM. Callus was induced on B5 medium with 0.1 mg/l benzyladenine (BA) and 3 or 5 mg/l naphthalene acetic acid in the dark. Shoot initiation occurred on modified Woody Plant medium (mWP) containing 0.5 mg/l BA, 500 mg/l polyvinylpyrrolidone and 10% (v/v) coconut milk. Multiple shoots were also regenerated directly from hypocotyl segments of 4 to 6 week old seedlings on B5 medium with 0.5 mg/l BA. Regenerated shoots could be rooted with 100% efficiency on mWP medium containing 0.5 mg/l indolebutyric acid and transferred to soil in the greenhouse. Suspension cultures were obtained from the callus using B5 medium with 0.5 mg/l 2,4-dichlorophenoxyacetic acid. Callus clumps grew from less than 1 mm to 4-6 mm in diameter within two weeks on transfer to shoot regeneration medium but failed to form shoots or somatic embryos.
  • Authors

  • Subbaiah, MM
  • Minocha, Subhash
  • Status

    Publication Date

  • November 1990
  • Has Subject Area

    Published In

  • Plant Cell Reports  Journal
  • Digital Object Identifier (doi)

    Pubmed Id

  • 24227057
  • Start Page

  • 370
  • End Page

  • 373
  • Volume

  • 9
  • Issue

  • 7