Changes in polyamines, inorganic ions and glutamine synthetase activity in response to nitrogen availability and form in red spruce (Picea rubens).

Academic Article

Abstract

  • We analyzed effects of nitrogen availability and form on growth rates, concentrations of polyamines and inorganic ions and glutamine synthetase activity in in-vitro-cultured red spruce (Picea rubens Sarg.) cells. Growth rates, concentrations of polyamines and glutamine synthetase activity declined when either the amount of nitrate or the total amount of N in the culture medium was reduced. When total N in the medium was increased, cell mass increased without significant changes in glutamine synthetase activity or polyamine concentration. Reductions in the amount of nitrate or total N in the culture medium resulted in increased accumulations of Ca, Mn and Zn in the cells, and K accumulation decreased in response to decreasing nitrate:ammonium ratios. The data indicate that changes in total N availability as well as the forms of N play important roles in the physiological responses of in-vitro-grown red spruce cells that mimic the observed responses of forest trees to soil N deficiency and N fertilization.
  • Authors

  • Serapiglia, Michelle J
  • Minocha, Rakesh
  • Minocha, Subhash
  • Status

    Publication Date

  • December 2008
  • Published In

  • Tree Physiology  Journal
  • Keywords

  • Cells, Cultured
  • Culture Media
  • Glutamate-Ammonia Ligase
  • Ions
  • Nitrates
  • Nitrogen
  • Picea
  • Plant Proteins
  • Polyamines
  • Potassium Compounds
  • Digital Object Identifier (doi)

    Pubmed Id

  • 19193562
  • Start Page

  • 1793
  • End Page

  • 1803
  • Volume

  • 28
  • Issue

  • 12