Template mixing: a method of enhancing detection and interpretation of codominant RAPD markers.

Academic Article

Abstract

  • Ten codominant RAPD markers, ranging in size from about 300 to about 1350 bp, were identified in mapping populations of chickpea (Cicer arietinum L.) and diploid strawberry (Fragaria vesca L.). A distinguishing feature of all ten markers, and perhaps of codominant RAPD markers in general, was the presence in heterozygous individuals of a non-parental, heteroduplex band migrating more slowly than either of the respective parental bands. This non-parental band could also be generated by mixing parental DNAs before PCR (template mixing). As a means of identifying primers likely to detect codominant RAPD markers, parental and mixed-template (parent-parent) PCR-product gel lanes were compared for 20 previously untested RAPD primers (10-base oligomers). Four primers that produced a total of five non-parental, heteroduplex bands in mixed-template reactions were selected, and then used to detect a total of five segregating, codominant markers and nine dominant markers in the respective F2 mapping population, a codominant marker frequency of 35.7%. When closely migrating fast and slow bands of codominant RAPDs were difficult to differentiate, parent-progeny template mixing was used to deliberately generate heteroduplex bands in fast- or slow-band F2 homozygotes, respectively, allowing confirmation of marker phenotype.
  • Authors

  • Davis, Thomas
  • Yu, H
  • Haigis, KM
  • McGowan, PJ
  • Status

    Publication Date

  • September 1995
  • Has Subject Area

    Published In

    Keywords

  • CODOMINANT
  • GENOME MAPPING
  • HETERODUPLEX DNA
  • RAPD MARKERS
  • TEMPLATE MIXING
  • Digital Object Identifier (doi)

    Pubmed Id

  • 24169884
  • Start Page

  • 582
  • End Page

  • 588
  • Volume

  • 91
  • Issue

  • 4