A genetic linkage map of microsatellite, gene-specific and morphological markers in diploid Fragaria.

Academic Article

Abstract

  • Diploid Fragaria provide a potential model for genomic studies in the Rosaceae. To develop a genetic linkage map of diploid Fragaria, we scored 78 markers (68 microsatellites, one sequence-characterised amplified region, six gene-specific markers and three morphological traits) in an interspecific F2 population of 94 plants generated from a cross of F.vesca f. semperflorens x F. nubicola. Co-segregation analysis arranged 76 markers into seven discrete linkage groups covering 448 cM, with linkage group sizes ranging from 100.3 cM to 22.9 cM. Marker coverage was generally good; however some clustering of markers was observed on six of the seven linkage groups. Segregation distortion was observed at a high proportion of loci (54%), which could reflect the interspecific nature of the progeny and, in some cases, the self-incompatibility of F. nubicola. Such distortion may also account for some of the marker clustering observed in the map. One of the morphological markers, pale-green leaf (pg) has not previously been mapped in Fragaria and was located to the mid-point of linkage group VI. The transferable nature of the markers used in this study means that the map will be ideal for use as a framework for additional marker incorporation aimed at enhancing and resolving map coverage of the diploid Fragaria genome. The map also provides a sound basis for linkage map transfer to the cultivated octoploid strawberry.
  • Authors

  • Sargent, DJ
  • Davis, Thomas
  • Tobutt, KR
  • Wilkinson, MJ
  • Battey, NH
  • Simpson, DW
  • Status

    Publication Date

  • November 2004
  • Published In

    Keywords

  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Plant
  • DNA Primers
  • Diploidy
  • Fragaria
  • Genetic Markers
  • Microsatellite Repeats
  • Digital Object Identifier (doi)

    Pubmed Id

  • 15290052
  • Start Page

  • 1385
  • End Page

  • 1391
  • Volume

  • 109
  • Issue

  • 7