Agrobacterium-mediated transformation of `Alpine' Fragaria vesca, and transmission of transgenes to R1 progeny.

Academic Article

Abstract

  • Agrobacterium-mediated transformation was used to stably introduce β-glucuronidase (gus) and neomycin phosphotransferase (nptII) marker genes into `Alpine' Fragaria vesca FRA 197, a diploid (2n = 2x = 14) strawberry. R0 generation transformants derived from a single clump of kanamycin-resistant callus were vegetatively propagated. The presence of the gus and nptII genes in five clonal R0 runner plants was confirmed by PCR. Southern analysis suggested two sites of nptII insertion. When R1 generation seedlings obtained via self-fertilization of R0 plants were tested by histochemical assay, 591 were GUS positive and 39 were GUS negative. The R1 segregation data fit a 15 : 1 ratio (0.5 > P > 0.25), consistent with the independent segregation of two transgene insertion loci. These results demonstrate the suitability of `Alpine' F. vesca for transgene research in strawberry.
  • Authors

  • Haymes, KM
  • Davis, Thomas
  • Status

    Publication Date

  • February 1998
  • Has Subject Area

    Published In

  • Plant Cell Reports  Journal
  • Keywords

  • Key words PCR
  • Progeny-analysis
  • Strawberry
  • Transformation
  • Digital Object Identifier (doi)

    Pubmed Id

  • 30736606
  • Start Page

  • 279
  • End Page

  • 283
  • Volume

  • 17
  • Issue

  • 4