Presence of trifoliin A, a Rhizobium-binding lectin, in clover root exudate.

Academic Article


  • Trifoliin A, a Rhizobium-binding glycoprotein from white clover, was detected in sterile clover root exudate by a sensitive immunofluorescence assay employing encapsulated cells of Rhizobium trifolii 0403 heat-fixed to microscope slides. Its presence in root exudate was further examined by immunoaffinity chromatography. The binding of trifoliin A to cells was specifically inhibited by the hapten, 2-deoxyglucose. Significantly higher quantities of trifoliin A were detected in root exudate of seedlings grown hydroponically in nitrogen-free medium than in rooting medium containing 15 mM NO-3, a concentration which completely suppressed root hair infection by the nitrogen-fixing symbiont. The presence of trifoliin A in root exudate may make it possible for recognition processes to occur before the microsymbiont attaches to its plant host.
  • Authors

  • Dazzo, FB
  • Hrabak, Estelle
  • Status

    Publication Date

  • 1981
  • Keywords

  • Chromatography, Affinity
  • Flavonoids
  • Fluorescent Antibody Technique
  • Lectins
  • Plant Lectins
  • Plants
  • Quercetin
  • Rhizobium
  • Digital Object Identifier (doi)

    Pubmed Id

  • 7029001
  • Start Page

  • 133
  • End Page

  • 138
  • Volume

  • 16
  • Issue

  • 2