DNA sequences from formalin-fixed nematodes: integrating molecular and morphological approaches to taxonomy.

Academic Article

Abstract

  • To effectively integrate DNA sequence analysis and classical nematode taxonomy, we must be able to obtain DNA sequences from formalin-fixed specimens. Microdissected sections of nematodes were removed from specimens fixed in formalin, using standard protocols and without destroying morphological features. The fixed sections provided sufficient template for multiple polymerase chain reaction-based DNA sequence analyses.
  • Authors

  • Thomas, William
  • Vida, JT
  • Frisse, LM
  • Mundo, M
  • Baldwin, JG
  • Status

    Publication Date

  • September 1997
  • Has Subject Area

    Published In

    Keywords

  • DNA sequence
  • formalin
  • microdissection
  • molecular biology
  • nematode
  • polymerase chain reaction
  • taxonomy
  • Start Page

  • 250
  • End Page

  • 254
  • Volume

  • 29
  • Issue

  • 3