High direct estimate of the mutation rate in the mitochondrial genome of Caenorhabditis elegans.

Academic Article

Abstract

  • Mutations in the mitochondrial genome have been implicated in numerous human genetic disorders and offer important data for phylogenetic, forensic, and population genetic studies. Using a long-term series of Caenorhabditis elegans mutation accumulation lines, we performed a wide-scale screen for mutations in the mitochondrial genome that revealed a mutation rate that is two orders of magnitude higher than previous indirect estimates, a highly biased mutational spectrum, multiple mutations affecting coding function, as well as mutational hotspots at homopolymeric nucleotide stretches.

    • Authors

  • Denver, DR
  • Morris, K
  • Lynch, M
  • Vassilieva, LL
  • Thomas, W. Kelley

    • Status

    Publication Date

  • September 29, 2000

    • Has Subject Area

    Published In

  • Science  Journal

    • Keywords

  • Amino Acid Substitution
  • Animals
  • Base Composition
  • Base Pairing
  • Caenorhabditis elegans
  • DNA, Helminth
  • DNA, Mitochondrial
  • Evolution, Molecular
  • Frameshift Mutation
  • Genome
  • Mitochondria
  • Mutagenesis, Insertional
  • Mutation
  • NADH Dehydrogenase
  • Phylogeny
  • Point Mutation
  • RNA, Transfer, Amino Acid-Specific
  • Repetitive Sequences, Nucleic Acid
  • Selection, Genetic
  • Sequence Deletion

    • Digital Object Identifier (doi)

    Pubmed Id

  • 11009418

    • Start Page

  • 2342

    • End Page

  • 2344

    • Volume

  • 289

    • Issue

  • 5488